FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_292_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3327448
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAAT2155.8398655E-817.8418081
CGGATAG1452.7303444E-416.6503853
GTCGCAT3351.6734703E-1015.6146091
TCGACCG1808.113645E-515.32869431
CCGGCTA1656.7075953E-414.7944471
CGGGTTA1951.6013999E-414.15211756
CTCGTAT5250.013.9490491
CGGGCTA1550.007558348613.4990821
CGACCGT1800.001407811313.41260832
TCCGTTA3102.8085196E-713.3692822
GTTCGAC2651.1181966E-513.01629829
CGAATAT2406.391271E-512.9337813
CGGTTTA1900.002114568812.7068733
CGGCTAT2508.447106E-512.5541451
CCGGAAT4751.8371793E-1012.4807281
GTCGTAA2551.0164411E-412.3079851
GTCCGGA4551.2369128E-912.26291
GTTCGAT4702.062734E-911.8715321
GTCCGTT3651.8038954E-611.46497251
GTCGGTA3054.224252E-511.4336471