FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_291_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_291_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29827891
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT34400.07.92276549
TCTCGAT36500.07.844546348
GATTCCG37500.07.3583252
GGCGGTA38250.07.213567759
CGGGAAT45600.06.9651931
ATTCCGT40700.06.94921653
CGGTACA41150.06.706166761
TCGATTC41500.06.650434550
GCGGTAC45250.06.47902160
GTCTAAG48050.06.46128144
GTTTATT186900.06.42400261
TTCTCGA43000.06.41737747
GTTTAAT134250.06.31754881
GTTAAAT137250.06.23031951
GTTTTTT381600.06.228671
GGGAAAT180650.06.22120481
GGAAAAT286550.06.13884541
TCACCCG59200.05.885133
GTTTGAT174200.05.77033331
AATCGAA40400.05.72069935