FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_290_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_290_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3782359
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGGC5359.094947E-1212.248939511
CGGTACA4401.15869625E-811.75996861
GATCGCG2500.00129950311.0385455
TCGTTGA5958.276402E-1010.43408152
CAACTAG6503.583409E-1010.0812859
CTAGGCT6951.20599E-99.42903912
CACGACC7901.3460522E-109.171617563
CGGGAAT5603.368972E-68.723661
GACGCCT10300.08.70540224
CTCGATT3600.0026886028.62386249
AACTAGG8051.8644641E-98.56859910
AGACGCC10501.8189894E-128.53958523
GCTCGTT7851.07356755E-88.34921750
GCGGTAC6756.597711E-78.17594260
CAACCGA4250.00132202838.1170054
CGTTGAA7706.872688E-88.06269853
TCTCGAT4450.00208208457.751376648
CCAACTA14150.07.5558088
CCTAACT9656.7557266E-97.509364628
GGCGGTA5552.87896E-47.45739159