FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_290_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_290_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3782359
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG1704.465619E-922.31563831
CGACCGT2053.846253E-818.50516332
GTCGCTT4500.017.0516321
GTTCGAC2402.3181929E-715.8092129
GTCGGTT3001.02209015E-815.1139461
GGCGTAT3851.3460522E-913.5889641
CGGTTAT2405.7549398E-513.0793771
CTTCGAT5501.8189894E-1212.6830331
GTCGGGT4751.8371793E-1012.4827741
GTCGCAT2801.7573991E-512.456551
CCCTAGT2305.889408E-412.1315971
GTCCGAA3751.9029176E-712.0911581
TCGACGC3451.0441909E-611.99702643
GTCCGTT4551.6159902E-811.4983531
GTTCGGA4309.126961E-811.3557391
TCGGGTA3359.702922E-611.3409962
GACCGTC4602.2435415E-811.24572733
AAGTTCG3752.8473823E-611.03770327
TAGACGG2200.006329622610.9736827
GCCGATA2550.001398155110.9422241