FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_289_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_289_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14657854
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT16400.012.20124249
GATTCCG17200.010.4276952
TCGATTC19750.010.13166350
TCTCGAT17900.010.02171548
TTCTCGA20950.09.38498747
ATTCCGT20700.08.49789853
GCGTAGT11153.6379788E-128.04216818
ACTAGGC16400.07.991462711
TTGCGTA12950.07.98989316
CGGGAAT21800.07.8442051
CGGTACA19450.07.803469761
GTTTATT93300.07.5931811
CGATTCC25900.07.590786551
TCACCCG26250.07.4900113
GTTTAAT67450.07.0883961
GGAAAAT137600.07.02539441
CGCGGTA7452.3203518E-56.94423617
GTTGCGT15550.06.87557315
GTAGTCG12605.18412E-106.843195457
TCGTCGG13706.91216E-116.79884248