FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_289_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14657854
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAT10950.014.6546541
GTCCGCT17900.012.6675371
GTCGAAT11700.012.2244651
GTCGCTT22250.011.6020181
GTCGGTT13200.011.36387351
GTCGATT17900.011.1084551
GTCCGGA20800.011.0691041
GTCCGAA14850.011.0408691
GTCCGAT17850.010.748711
TCGACCG6755.456968E-1210.72973331
GTCGTTT27950.010.4840561
CGCTAGT4703.1091622E-710.3911111
GTCGCCT13850.010.3268041
GTCGGCT22950.09.8801271
GTCGGGA20650.09.7980531
CGACCGT7801.0913936E-119.72743932
GTCGTAA12600.09.6901241
GTTCGAT23800.09.6738391
GTTCGGT21300.09.6628281
CGATCGG9650.09.65086241