FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_280_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_280_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3671365
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA6050.012.54242861
CGCGCTA2200.006332574410.9729823
GGCGGTA6705.456968E-1210.8099459
CGCGCAT2300.00880429310.49660959
GCGGTAC7801.8189894E-1210.16979160
GTCTAAG6554.110916E-1010.00644644
CAATCGG4057.956952E-59.3696414
GGGCGGT8354.1836756E-119.08700958
TTTACGC3100.0077189388.9009567
CTCGATT4704.148198E-58.80854649
ACACACG9457.2759576E-128.7600352
TCGACTG5151.2815037E-58.7065187
GTTAACT11052.0008883E-117.89398861
ATTCCGT5704.5746096E-57.86662953
AATCGAA3950.00620768367.8593985
TCACCCG7503.864934E-77.81875233
GATTCCG5755.0997798E-57.79833152
ACACGGG9751.0149961E-97.78337354
TAGCATA10203.110472E-107.7770059
TCTCGAT5856.311545E-57.66586148