FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_280_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_280_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3671365
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG2057.4578566E-1121.87112631
CCGACAA1704.531217E-516.4098421
GTCGGTT3351.6734703E-1015.6138421
CGACCGT2756.6122084E-815.04957732
GTTCGAC3001.9141953E-713.796761529
GTCGGAT4901.8189894E-1213.5213751
GGCGTTT5450.013.4364991
TCCGATC4153.3469405E-1013.3194912
GTCCGCT5001.8189894E-1213.2509481
GTCGGCT6200.012.9359841
AAGTTCG3551.0775693E-712.63083827
CCCGTAC4154.14002E-912.6039461
GTCGATT3701.6024387E-712.2519431
GTCCGTT4358.3327905E-912.0244531
GTCGAGA2357.0686406E-411.870951
AGTTCGA3802.6120506E-711.79986228
TCGGATT5401.4733814E-1011.5158092
GACGACC2701.9194315E-411.49620432
TTCGACC3652.0597472E-611.33933930
CTCCGAT8000.011.3330471