FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_279_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_279_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31292096
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT36450.010.69523849
CGGTACA50600.09.6121261
GGCGGTA51550.08.9652659
TCTCGAT44750.08.86507648
TCGATTC46500.08.68045450
GATTCCG45300.08.60403852
ATTCCGT48700.08.35738353
GCGGTAC57100.08.27540460
GTCTAAG58900.08.08222744
TTCTCGA47550.08.05198447
CGCTAGT13100.07.900350633
GTTTAAT147650.07.11495261
TAGCATA81800.06.95662559
TCACCCG61800.06.92119363
ACGGGCG72800.06.91670356
CGATTCC60150.06.65183951
GGAAAAT296700.06.5873311
CCGGACA60650.06.539941312
CATATGC88550.06.46586112
CCCGGAC67650.06.37296311