FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_279_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31292096
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG18900.015.69291431
CGACCGT21050.014.74535832
GTCCGTT40800.012.9939851
GTTCGAC24050.012.33397629
GTCGGTT28500.011.2590551
GTCGGAT35450.011.117861
TCGACGC27650.011.10186243
CCCGTAT28400.010.8074511
GTCCGAT33650.010.7797141
GTCGCAT26450.010.6811581
TTCGACC28900.010.50204830
GTCCGCT41750.010.1920691
GTCCGGA40400.010.1873121
GTCCGGT44150.010.1120271
GCCGTAT28800.010.0518171
CCCGAAT36650.09.9925031
GTCCAGT155000.09.991031
GTCGCGA8201.8189894E-129.783021
GTCGATT29600.09.7801461
GTCGGCT48450.09.7184931