FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_278_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_278_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5474612
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG2100.004465349511.49995963
GGCGGTA8600.011.22882559
CGGTACA8300.011.22080161
GCGGTAC9650.09.65016960
GTCTAAG10250.09.42435644
CGCGGTA2950.0051371789.35324917
TTGCGTA4805.329911E-58.62252616
CTCGATT6151.526998E-68.41475849
GCGTAGT4653.571237E-48.15857718
GTTGCGT5502.9310318E-58.15213215
TCGTCGG5603.6586658E-58.008948
GTAGTCG5351.8870493E-47.73546257
TCGAAAC5805.6800367E-57.731317561
ACACACG14550.07.58517652
GGGGAAT20550.07.47246741
ACACGGG14050.07.364839654
AATCGAT5200.00117735037.29745055
GGGCGGT12304.0017767E-117.290258458
ACGGGCG13002.0008883E-117.162740756
CGTCGGC6855.758989E-57.05119949