FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_278_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5474612
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG2952.910383E-1117.5363731
CGACCGT3705.4569682E-1114.91367532
CCCGTAT4051.2732926E-1114.6447851
GTTCGAC3951.5643309E-1013.97066529
GCCGTAT5400.013.5679641
CTCGAGT6700.011.4561061
TTCGACC4904.7220965E-911.26175930
TCGACGC4602.2413587E-811.24676543
GGCGTTT9350.010.8212021
GTCCGTT7101.8189894E-1210.8106911
GTCGGAT6805.456968E-1210.7745591
GTCCGGA7550.010.6284511
TCTCGAC5101.01850674E-710.14506841
GTCCGCT6908.0035534E-1110.1127681
GTCGACT5552.7497663E-810.05810451
GCGATCG4159.553936E-69.97403840
GTCGCTT7001.05501385E-109.9683011
AAGTTCG5201.3542194E-79.94906227
CTCGTAT7757.2759576E-129.9039891
GTCCATT28200.09.8976021