FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_277_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_277_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31226004
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA51550.011.64324561
GGCGGTA57550.010.06831659
GTCTAAG61150.09.70308344
CTCGATT42450.09.58985449
GCGGTAC64850.09.41398760
GATTCCG46300.09.0139752
TCTCGAT45850.08.80311148
TTCTCGA48450.08.68584147
TCGATTC48250.08.58008650
GGGCGGT76900.08.02826558
ACACACG77400.07.9767152
TACATCG60700.07.8992493
ATTCCGT48950.07.891729453
TCACCCG60500.07.8113293
TCGACTG44650.07.80188947
ACGGGCG81400.07.7114256
CGATTCC58900.07.554122451
TAGCATA94550.07.40481579
GCGTCGT42250.07.34662519
ATCGACT51450.07.3072546