FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_277_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31226004
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG18700.015.49217131
CGACCGT22000.013.63855732
GTTCGAC25200.012.45568729
GTCCGTT40050.011.7565771
GCCGTAT31050.010.8958161
GTCGGTT27950.010.856431
CGATCGG23050.010.47506441
TCGACGC30450.010.42074643
GTCGGAT43500.010.2629051
CCCGTAT24150.09.9650971
GTCCGGT51850.09.9554881
GTCCGAT38600.09.9392821
GTCGGAA51800.09.8977661
GTCGTTT53250.09.8902431
GTCGAAT28150.09.7880971
GTCCGCT38150.09.6908291
TTCGACC31450.09.65079530
AGTTCGA33800.09.59262128
GTCCGAA30000.09.3007581
GTCGCTT40750.09.0725181