FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_276_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_276_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3228475
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCGT4005.0740164E-711.20997853
TCTCGAT3853.895306E-610.7526648
TCGATTC3955.2889827E-610.48109150
CTCGATT3652.5381689E-510.39733749
GATTCCG4251.267695E-59.73898552
TTCTCGA4401.9099558E-59.40770247
GCTCGAT3500.00187860838.9711631
GTCTAAG6001.0717249E-68.62452944
GGCGGTA5857.0954356E-68.25404259
GAATCGA4250.00132259198.1165754
CGGTACA6009.943013E-68.04881361
ACGCCCT4805.0058396E-47.90594169
GTTTAAT16500.07.8233361
CCGGACA5805.6835088E-57.7306612
CGAAAAT4550.0023161857.66765931
CGATTCC5402.0970768E-47.664942351
TAGCATA9201.7770253E-77.12262449
AATTCAA17600.07.0555595
TCACCCG6452.1096536E-46.9522473
CGGAGAT7552.3787228E-56.9313611