FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_276_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_276_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3228475
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAT2807.1824616E-814.9481891
GTCCGTT3858.731149E-1114.4952141
CGGGCTA1450.00485254914.43273451
CGATCGG1750.001137538513.79692241
GTCGGAT3801.1059456E-913.7680681
GTCGCTT4659.094947E-1213.5015911
GTCGGTA2851.426386E-613.4621131
CGCCAAT2608.192164E-613.4150421
CCCGTAT2405.753235E-513.0796661
CCCGAAT3002.5374648E-612.7890071
GTCCGCT3601.1178054E-712.5952341
GGCGAAT2254.893506E-412.4014621
CTCGGTT4802.1827873E-1012.3530181
GCGACTA2000.00307407912.0885952
GCGATCG2357.746596E-411.74206140
TCGCATA2651.5913579E-411.7301732
ACGCGTC3853.0795127E-711.65090367
GGCGTAT3601.5244095E-611.6263691
CCCTAAT4652.226443E-811.2513261
GTCGGCA6251.2732926E-1111.1613151