FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_275_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_275_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6343077
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGA5201.3455065E-79.95354866
CCGCTAG4957.3496267E-79.758553565
CTCGATT8557.2759576E-129.28163149
GATTCCG9700.09.24565152
CGCTAGT3050.00674768639.04823233
ATTCCGT9801.8189894E-128.79926653
TTCTCGA9651.0913936E-118.57954747
TCTCGAT9456.002665E-118.39707148
AATCGGA8252.9449438E-98.3625625
TCGATTC10507.2759576E-128.2151150
GTTTAAT30900.07.90384151
GTTTTTT84800.07.85843751
CGGTACA9656.8102963E-97.506630461
TTTCTCG11052.1282176E-107.492545646
GTTTTCG7402.6432808E-67.457207710
GGCGGTA9758.336428E-97.428467359
TCACCCG12609.094947E-127.39184953
GTTAGAT16600.07.35628651
CGAAGAT11001.198714E-97.29514361
ACGTCTA5300.00144438817.15821610