FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_275_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6343077
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG4300.018.44751231
CGACCGT4450.017.82568632
GTTCGAC5400.015.32969729
GTCGTTA5150.014.8988011
CGATCGG4700.013.9443841
TCGACGC6000.013.79640143
TTCGACC6100.013.57033730
GTCCGAA6200.012.3756171
CCGGTAT5501.2732926E-1112.0483281
GTCGGTT6400.011.9888791
GTCGTAT6301.8189894E-1211.625581
GTCCGTT8100.011.6255791
GTCGGCT10000.011.5093241
GATCGCG2701.9170724E-411.49799740
AAGTTCG7550.011.42113127
TCTCGAC6950.011.415277541
GCGATCG5451.7826096E-1011.39251140
AGTTCGA7300.011.33977528
GTTCGTT10800.010.9797131
GTTCGAT8600.010.9496741