FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_274_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_274_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30555158
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT37800.013.14300549
GATTCCG43750.011.66806652
TCTCGAT44400.010.87769948
TCGATTC49800.010.11457250
TTCTCGA50050.09.85562447
ATTCCGT50250.09.74682653
CGGTACA50250.09.12971261
TCACCCG61200.08.9617963
GTCTAAG56550.08.84551544
CGATTCC60700.08.69395251
CCGGACA61250.08.221477512
GGCGGTA56050.08.18402859
CCCGGAC63250.08.179511
GCGGTAC61850.07.639897360
TTTCTCG67750.07.33171246
GTAGTCG29400.07.273222457
CGTGGGT70400.07.2015757
ACCCGGC83450.07.1097725
ACTAGGC37500.07.081991711
GTTTAAT139200.07.0696911