FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_274_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30555158
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG19800.017.24419831
CGACCGT22400.015.55054632
GTTCGAC23750.014.81355529
GTCCGTT37500.012.9294321
GTCGGTT26500.012.3730821
TCGACGC31700.011.64212743
TTCGACC31650.011.00635130
TCTCGAC34350.010.84509241
AGTTCGA33900.010.58170528
TCGCGTA13150.010.49481966
AAGTTCG33650.010.45531527
GTCGCTT41450.010.3508621
GTCGAGA21750.010.2641
CGCGTAA14250.010.16977767
GTCCGAT39850.09.8911331
GTCCGGA41200.09.8210221
GTCGTTT54150.09.7268991
GTCGGAA51800.09.6294651
CGATCGG26200.09.61076241
CGCGTAT6301.9415893E-89.4124851