FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_273_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_273_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2707149
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA1450.005206860614.2801732
CGAAATT1950.00257146912.3798888
CGAATTA2409.245016E-411.49688458
GTTGCGC2150.005316518711.23366529
ATCGACT4301.4574209E-59.6249436
TTGACGT3050.00675788949.04622657
TCTAGGT3500.0020855258.8685347
CGAGAAT3150.0079757078.8653321
TACATCG5704.9921073E-68.472643
TCGACTG3750.00389472868.2772987
CGAAGAT4804.410805E-47.9995771
CTTAAAT13950.07.75716541
CCCGAAT4500.00206638087.75716451
ACACACG6251.7144543E-57.726762352
GTCTAAT4600.002563957.5885311
GTTACAT11051.6370905E-107.58166361
GATTCCG4150.00965264257.4807351
GCTAAAT9151.3072713E-77.24849841
GTTAAAT11705.7298166E-107.160461
CTTCAAT11857.548806E-107.06982141