FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_273_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2707149
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG1552.2542254E-517.800631
GTCGGTT3001.0193617E-815.1165141
GTCGGAT4051.2732926E-1114.6427771
CGACCGT1901.286238E-414.5215432
GTTCGAC1951.6025278E-414.15076229
GTCGCAA1500.006069729613.9537061
CGTGCGA1750.001137410413.79699237
CCCGAAT3556.2755134E-913.7571751
TTCGACC2355.2438907E-513.20939830
TCGAAAC2153.6658804E-412.8334647
CCCGATT2254.8869586E-412.4032951
GTCCGCT3407.6301694E-712.3120941
GTCGGCT4255.864422E-912.3120941
CCGGAAT3459.1034417E-712.1336581
CGATCGG2000.003103646412.07303841
TACGACG2050.003737540711.7768349
CCGTAAT2408.4061956E-411.6280891
CTCGTTT7050.011.3806821
GTTCGGT4004.383146E-711.3373861
GTCCAAT7400.011.3138161