FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_250_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_250_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4110495
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT5100.013.52951949
GATTCCG6000.012.64717652
TTCTCGA5750.012.59887447
ATTCCGT6400.011.85658353
TCTCGAT6503.092282E-1110.61546948
ACTAGGC5007.6255674E-810.34756310
TCGATTC7252.3646862E-119.99318350
CGGTACA7302.7284841E-119.92328661
GTCTAAG7901.2732926E-119.6073244
TCACCCG8405.456968E-129.4451243
CGATTCC8052.0008883E-119.42646651
TCGTTGA6101.4083162E-79.0471651
GGCGGTA7759.113137E-108.90091359
TTTCTCG8303.583409E-108.72813546
CCCGGAC9901.8189894E-118.36197311
GCGATAG3750.00389112868.27835312
GTTCAAT10557.2759576E-128.2681921
TAGCATA10901.8189894E-128.2272219
GCGGTAC8404.129106E-98.21245260
TTCGGCG5553.2793603E-58.07917938