FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_250_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_250_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4110495
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT2700.022.99290532
TCGACCG2750.022.5748531
GTTCGAC3700.017.71204229
CCCGAAT5500.015.218171
TTCGACC4350.015.06523130
AGTTCGA5050.015.02616328
TCGACGC4151.8189894E-1214.959784543
CGCGAGT1901.2858743E-414.52236543
CTCGACG4850.014.223842
TCTCGAC4651.0913936E-1113.35201841
GTCGTTA2901.7370749E-613.2284391
AAGTTCG5003.6379788E-1213.10691327
TCGCGTA1850.001726128413.05463266
CGCTAAT2456.9883E-512.8112151
GCCGACC5501.8189894E-1212.54600965
GATCGCG2204.448308E-412.54280540
GACCGTC5351.0913936E-1112.24855633
CCGAATC4359.595169E-911.9107022
GTCCGAA4409.855285E-911.8891951
CGGATCG2651.611847E-411.71422138