FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_249_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_249_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11063295
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCCG12750.012.17332152
CTCGATT14800.011.4220849
ATTCCGT16450.010.0642253
TTCTCGA16450.09.64557547
TCGATTC17250.09.3998250
CGGTACA16250.09.34017861
TCTCGAT16450.09.01741348
GGCGGTA17850.08.69503259
TCACCCG21050.08.5213623
CGCTAGT4502.5005412E-48.43286733
CGATACG10901.8189894E-128.23016829
CGATTCC19000.08.16890151
GTCTAAG19800.07.665930744
GCGGTAC20500.07.571280560
CGATAGT4850.00432263137.1939731
TTTCTCG24150.07.141493346
ACACACG26450.06.78085452
CCGGACA22800.06.656082612
AACTCAC41150.06.6225145
TAAATCG8401.7339808E-56.5705013