FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_249_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_249_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11063295
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG5800.017.83898531
CGACCGT6500.016.9789832
CGATCGG6850.013.59550241
GTTCGAC8400.012.72918329
GTCCGTT13200.012.6815391
GTCCGAT14050.012.1625421
GCGATCG8550.011.29577340
TTCGACC9200.011.24692630
CCCGAAT13350.011.2328971
TCGACGC10300.011.05042943
AGTTCGA10950.011.02483428
GTTCGGA11200.010.8981961
GGCGTAT12000.010.7528871
GTCGGAT14600.010.7489061
ATTCGAA9750.010.2583658
GTCGCTT16400.09.9944441
GTCCGCA16400.09.9944441
GTTCGAT18500.09.8024861
GTCCAGT50750.09.7579131
CGTGCGA8950.09.63454437