FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_248_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_248_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5794894
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA2903.718565E-410.70463617
CTCGATT5907.1122486E-1010.525290549
CGCGTTA2300.00880309610.49696758
GGCGGTA7851.0913936E-119.665997559
CGGTACA7504.8930815E-109.19901161
GATTCGC4552.817384E-48.340055
GTTAAAT27650.08.1999681
TCGATTC7001.1407337E-67.885614450
GATTCCG6604.2022075E-67.83864552
GTTTAAT27900.07.6263991
GCGGTAC9152.0259904E-87.53919660
TCTCGAT6958.703806E-67.445885748
ATTCCGT7553.569312E-67.3090253
TTCTCGA7151.3002178E-57.236796447
GTTTGAT35300.07.2134341
GTTTAAC18900.07.19774961
GGAAAAT53000.07.1737221
GTCTAAT9805.828224E-87.11865331
GATCCAA20100.07.03679945
GTTCAAT17400.07.01637361