FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_248_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5794894
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG2553.6379788E-1220.28693631
GTCGGTT6550.015.4346751
GTTCGAC3951.5643309E-1013.97166729
CGTATTA3651.0886652E-813.2283093
CCGACAA3701.1330485E-813.1906771
CGACCGT4003.911373E-812.07072732
TCGACGC4601.7498678E-911.99678943
CCCGAAT6251.8189894E-1211.7133221
GTCGAGT4501.3831595E-811.6203591
CGCCATT7600.011.467461
GTCGATA3358.752651E-611.446921
GTCCGAT5259.767973E-1011.2883481
GTCCGAA5401.5588739E-910.9747831
GTCGGAT5753.783498E-1010.9130331
TTCGACC4905.675247E-810.55848930
GTCCGCT8050.010.3933641
GTCGTTT9450.010.3292081
GTCCGGA7453.6379788E-1210.2945461
GTCCAGT27450.010.2868751
AAGTTCG4703.6295023E-710.27437827