FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_247_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_247_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14026166
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT17950.09.80204849
CGGTACA24250.09.24570261
GGCGGTA23100.09.1075959
CGCTAGT6151.5288406E-68.41435933
GCGGTAC26800.08.23653960
TTCTCGA22700.08.05368247
GTTCGAT20150.07.96613361
GATTCCG22150.07.94160752
TACGCGA7051.2768196E-67.82721456
TCGATTC22950.07.81684950
GTTAAAT64950.07.7365641
TCTCGAT21650.07.648273548
ATTCCGT22100.07.647380453
GTCTAAG26650.07.378350744
CGCGGTA8001.0809945E-67.329128317
GCGTATA9655.4022166E-87.148494739
AATCGTA8402.326251E-66.98134145
CCGGACA27550.06.885729312
GTTTAAT64000.06.81545451
TCACCCG28850.06.81538723