FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_246_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_246_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28946770
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA50400.012.38802661
GGCGGTA52250.011.68369759
CTCGATT38250.011.63530149
ACTAGGC69300.011.29778911
GTCTAAG62050.010.618916544
GCGGTAC58050.010.57618660
TCTCGAT41300.010.35776948
ATTCCGT43700.010.18175353
TCGTTGA75300.09.66504852
ACGCCTC76400.09.660830525
TCGATTC47250.09.63810150
GATTCCG49450.09.62563452
CAACTAG90700.09.5443479
CTAGGCT79300.09.30780712
CGAAAAT45250.09.1776781
TCAACCG42400.09.0305193
ACCCATG132600.09.0258376
CAACCGA51250.09.0196524
CCCGGAC55650.08.92479211
GCTCGTT81600.08.87868150