FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_246_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28946770
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG19700.019.95814131
CGACCGT20650.019.20685432
GTTCGAC24750.016.16627729
TCGACGC28350.014.59943143
GTCGGTT20450.013.8138481
TTCGACC30250.013.79634330
CGATCGG20650.013.36318341
TCTCGAC30600.013.18877441
AGTTCGA32950.012.980494528
TAGACGC48250.012.72516539
CGACGAA49200.012.61799619
GCGATCG21450.012.54319240
AAGTTCG33800.011.9397227
GTCGTTT51150.011.8638751
GACCGTC35500.011.75534833
CGTGCGA22800.011.49750837
CTCGACG35300.011.43276142
GTCCGTT38550.011.3085941
GTCGCGA5602.382876E-1011.2100471
GCGACGA55250.011.04902618