FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_245_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_245_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6001043
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG6453.092282E-1010.1644229
GATTCCG6808.28004E-99.13077352
CATTCGA8102.0881998E-98.5173994
ACGGAAT8658.076313E-108.3750433
GTTCGAT6907.7102413E-78.0925741
TACGATA8152.082561E-88.04450327
CCCGTAT5653.5409787E-58.0299111
GATACGG9102.1664164E-97.96281530
CTCGATT7859.477117E-87.910909749
TCTCGAT7453.471432E-77.87184348
ACTAGGC9001.2101918E-77.281101711
TTCTCGA8101.3132139E-67.239185347
TCGATTC8604.3702494E-77.221004550
CGGTACA7755.2594623E-67.121584461
TACGGAA10706.5847416E-97.094654632
GTTAGAT16300.06.85138231
TCACTAA18300.06.7845238
CCTAGAT9401.5753158E-66.6828311
ATTCCGT9301.6014747E-66.67615253
CAACTCA26950.06.6558964