FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_245_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6001043
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTT6500.017.7082021
GTCCGTT8250.012.2607751
GTCCGGT10400.012.0737741
CCCGTAT4155.5768396E-811.7666761
GTCCGGA6501.8189894E-1211.2688561
CCGGAAA5804.3655746E-1010.8247631
GTCCGAT8700.010.8247621
TCCGAAC4854.7702997E-810.6842092
GGCGTTT9800.010.6774861
CGCCTAT2953.9281824E-410.6412921
TACTACG4956.581831E-810.45231554
CGCATAT3702.6148798E-510.3696681
GTCGTGA5805.0167728E-910.2233881
CGGCTAT4502.0007774E-610.0763841
GTCGCTT7757.2759576E-129.90136051
GTCCGAA5704.1731255E-89.7908181
CGCTTAT5007.2696275E-79.7663411
TCCGCTA4251.245223E-59.7540532
GTCGTTT10400.09.7260961
GTCGGAT9000.09.688831