FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_244_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_244_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7926364
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT8454.598405E-98.16567949
GATTCCG11651.8189894E-127.993840752
ATTCCGT11709.640644E-117.37007353
GTTTAAT35900.07.1933691
CGGGAAT11302.0972948E-97.1030431
TCTCGAT9959.4234565E-86.934322448
CGACAAT6208.943412E-46.7543661
TACCGAA6653.0745647E-46.74197779
GTTGAAT28050.06.7182461
TCGATTC11301.9643267E-86.71681350
GTTAAAT34450.06.58444551
GTTTATT51950.06.58317761
TACCCGA6300.00122605636.5691069
GGAAAAT73400.06.5135771
ATTCTCG7955.726907E-56.50762656
TTCTCGA10703.535988E-76.447417747
GTTTAGT23900.06.4246551
CGGTACA10301.255723E-66.363113461
GTTTGAA56050.06.22614771
CCGGACA14551.9699655E-96.16323612