FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_244_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7926364
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAT10000.013.9485341
GTCGCAT6600.013.2088381
GTCCGTT9700.012.582441
GTCCGCT10900.012.47691
GTCGGTT7550.012.4705441
GTCCGAA8000.012.2049671
GTCGGAA10000.011.8562541
GTCGACG5157.075869E-1011.5109251
GTCGAGA4903.974492E-911.3865591
GTCGTAT8550.011.0121
GTCCGAT8450.010.7296411
TCGACCG3602.1887214E-510.53831331
GGCGTAT8100.010.3322481
GTCGTTT13450.010.1113911
TACGACG4804.852809E-710.0592349
CGGCTTA3803.969294E-59.9839793
GTCGTGA6301.8444553E-99.9632391
CCCGAAT8400.09.9632381
CCCGTAT7051.2369128E-109.8925771
CGACCGT3905.2814037E-59.7275532