FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_243_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_243_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6527793
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT6251.9354047E-99.93534249
TAGCCGA3050.0067477159.0482354
TCGATTC8709.822543E-118.7235650
TCTCGAT8051.6823833E-88.14169348
GTTTCGA4100.0086619957.57237964
CGGGAAT10656.020855E-107.53794961
CGAAAAT8806.6100256E-87.53608751
GATTCCG9252.4798283E-87.45719452
GGCCGTT9402.6766429E-87.4263791
GTAGTCG4650.0032069877.41738657
TTCTCGA8503.607347E-77.304671347
CGGTACA9453.6703568E-87.30020961
CTTAGAT21000.07.1470061
GGCGGTA8907.7566074E-76.975836859
TAGTCGC5000.0064966216.89832858
CGACTGT7502.5472298E-56.89785159
GTTCAAC21200.06.75029851
GTTTATT43100.06.64066551
GAATCTA17450.06.5236734
GTTTAAT34550.06.46557141