FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_243_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6527793
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGCT8450.016.0958541
GTCCGGA8450.013.6195681
GTCCGTT8200.013.1842041
GTCGCGT3203.67354E-713.077881
GTCGCAT5650.012.9621471
CCCGTAT6200.012.9372581
GTCGAGT5601.8189894E-1212.4551241
CGATCGG3954.313115E-711.35225241
GTCCGAA6800.011.2828771
GGCCTAT6755.456968E-1210.8497971
GTCGTAT7151.8189894E-1210.7305681
GTCGAGA4351.298069E-610.4222181
GCCTAAT9050.010.4045021
CGCTAGT2700.002253030410.3331391
GGCGTTT10250.010.2071261
GTCGAAT5151.00046236E-710.15757751
GTCCGAT7453.45608E-119.8303531
GTCGGTA6051.00480975E-89.7994021
TCGAATT6453.259629E-99.63964752
TCGACCG3300.00122041839.40613331