FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_242_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_242_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072736
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA3451.6473503E-49.99823861
GTCTAAG3652.9010593E-49.45152944
GGCGGTA4057.973625E-59.36736259
AGCGAAC3050.00675379539.0467126
GCGGTAC4007.2552415E-48.62306360
TCACCCG4058.200826E-48.5174283
TCAATCG3800.00437990678.1700073
GTTTATT13150.07.96136141
ACACACG4805.0200697E-47.903521552
GGGCGGT4400.00186472767.838392758
AATCTAT7051.2706714E-67.8285965
TCTAAGT5805.67674E-57.731022445
GTTTAAC5906.072509E-57.68923571
GTTGAAT8502.9652074E-77.3900171
AGATTCA9851.0077201E-87.35474354
ATTGTAC8051.1874836E-67.28460555
GGGGAAT6754.0757797E-57.2379591
ACACGGG5250.00130416247.226948754
GATCTCC8604.3731598E-77.2198325
TAAACCC7201.4319106E-57.18657973