FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_242_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072736
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG850.00412720120.2890879
CCCGCTA1300.00234200616.1013091
CGACCGT1300.002528025315.91797732
GTCGTGA1901.1665067E-414.6889131
GTCCGAA1901.1665067E-414.6889131
GGCGAAT1708.383158E-414.3648941
ATACCGA1450.005225345514.27232313
GTCGTTT3052.0371044E-713.7257071
GTCGTAT2303.849108E-513.6511091
CCCGCCC8450.013.0609055
CTCGTAA2153.328131E-412.98091
GTCGGCT3559.282485E-812.7752181
GTTACGA2204.041017E-412.685881
GGCGTTT3601.1140946E-712.5977831
CCGGTTT2852.1014794E-512.2407611
CCCCGCC10300.012.054394
ACCGCAT2357.0404244E-411.87614251
GTCCGTA3256.191167E-611.8076281
GTTCGAT2752.0454336E-411.4172921
CCCCCGC11300.011.2928423