FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_241_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_241_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916469
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCCG4259.0525646E-811.36217252
TTACGCT2500.001301454711.0364048
GTCTAAG4501.9904292E-710.73241344
CTCGATT4209.4910865E-710.67857149
TCGATTC4552.3149732E-710.61538450
CAACCGA3401.4207422E-410.1461894
CGGTACA4451.9967829E-610.07744161
TCTCGAT4502.3026914E-69.96581248
ATTCCGT4603.0559222E-69.74749353
AACCGAT3602.527742E-49.5823475
TCACCCG5857.389281E-89.4344423
GGCGGTA4201.1854981E-49.0330859
GTTTAAT12050.08.9737231
GCGGTAC5051.0001631E-58.87890360
CAATCGG4906.7653484E-58.4482554
TCAATCG5755.6175595E-68.3987043
CGATTCC5755.6227454E-68.39812851
TTCTCGA4957.62658E-58.36205947
CAATGCG4150.00104658328.31082911
CGATACG3750.00387865588.281136529