FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_241_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_241_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916469
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGA1903.139412E-718.3479161
GTCGGTT2207.622293E-817.430521
TCGCGTG1503.4949245E-416.1195952
GTCGCGT2251.8397714E-615.49379351
TCGACCG1605.7761825E-415.08880331
GCCTAAT3901.6443664E-913.4080921
TCGCGTT1550.00804944513.3710932
CCCGAAT3153.0455158E-713.2803951
TTCGACC2406.390075E-512.93392630
AACCGGT1900.002109656612.71071963
GTCCGAA2751.4675794E-512.6767411
TCGTATA2357.65319E-411.7589762
GTCCGTT3953.7573045E-711.4732511
GCCCTAC3651.8108694E-611.46116351
GTTCGAC2150.00533019611.2298429
CGACCGT2150.005333601511.22887732
TCTCGAC2500.001299427111.03846441
GCCAGAT12700.010.9798551
GTCACGA4157.1131217E-710.9203251
TCCGAAC2853.123565E-410.9079972