FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_224_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_224_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4315489
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGAT2903.7131607E-410.7061925
CTCGATT4351.7313173E-48.72386749
GGCGGTA6051.2123073E-68.55107459
CGGTACA5857.094328E-68.25433661
GATTCCG5452.6125876E-58.22719552
TTCTCGA4855.60368E-47.8233247
GGGGAAT14750.07.56936171
GTCTAAG6857.0967744E-67.55407244
GTCGCCG4600.00288001357.499245664
GTTTTAT32350.07.33391671
GTTGAAT15350.07.2734911
GCGGTAC7151.3028735E-57.23560860
TCTCGAT5250.00130359197.227676448
GTTTAAT19500.06.97800541
TAGCATA11403.2177923E-96.95853579
CCGGACA7653.352059E-56.76324112
AATCCAA23000.06.7495555
TCGATTC6300.0012212186.571224750
ATTCCGT6400.00146327326.466974753
CTTAAAT21600.06.46111631