FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_224_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4315489
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGT1552.0522797E-517.9963991
CGCCAAT2605.0820745E-714.7518561
GTCCGAT4151.8189894E-1114.2832871
GTCGATA2253.1468742E-513.9472091
TCCGATC4254.8930815E-1013.0055172
GTCGCGT2254.9072254E-412.3975191
CGACCGT2306.47442E-411.99578232
CTCGTAT5309.094947E-1111.841971
CTACTCG3105.5821896E-511.1253774
CCTAGGT4401.259832E-711.0943721
GTCCGTT5753.783498E-1010.9152081
GTCCGGA5451.8080755E-910.8762651
GTCGGAT5158.6656655E-910.8327841
TCGACCG2600.001805694210.61165131
CCGGTTA2650.001931396910.52619651
GTCCAGT21200.010.52619651
GCCGATT4056.2821855E-610.3312661
GTTCGGT6908.185452E-1110.1066741
CTCGGAT7352.5465852E-119.9622931
GTCGTAT5007.3011506E-79.7630471