FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_223_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_223_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5535721
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA7951.5279511E-109.11133361
GGCGGTA8658.731149E-118.7719759
CTCGATT6951.1363227E-78.43955349
GTCGATT3750.00348542238.3800561
TCACCCG10751.8189894E-128.3429983
GCGGTAC9701.07320375E-108.1783660
GACGTAA4400.00186580997.83844641
TCTCGAT7705.861966E-77.617173748
TTCTCGA7553.555153E-67.310943647
TAGCATA14750.07.24857049
GTTTAAC18350.07.2307481
AATCTAC15550.07.0989875
TCGATTC7805.7682682E-67.077513750
ATTCCGT7856.3767275E-67.02995653
ACTTGCG5450.00194022066.961178310
AATCGAA7502.5393094E-56.8993285
CAATCTA9654.0678424E-76.7922084
CAATCGG5100.0078538396.76429245
GTTCGAT6753.1716313E-46.72473671
AATCCGA7201.10830006E-46.70768075