FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_223_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5535721
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTA1400.00413846414.78303745
GTCGGAT6650.014.1670041
GTCGTAT6250.013.9571221
TCGACCG3557.372364E-913.60120531
GTCCGTT7400.013.20268251
CGATCGG4205.824404E-912.3185341
GTCGGGA9200.012.1366281
GTCGGTT4903.0559022E-1012.1056671
GTCGAGA4451.1566954E-811.76162051
GTCGCTT7550.011.5539091
GTCGCTA4853.3505785E-911.5110281
CGACCGT4259.071846E-811.36100632
GTTCGAC4753.5934136E-810.89207629
GTCGGCT8650.010.8913961
GTCCCGT11250.010.855541
GTCCGAT5301.3413228E-810.5336771
GTCCGGT8650.010.4880111
GTACGAT5754.323738E-910.31613351
TTCGAAT4703.6343954E-710.2733439
CCGGTAT4451.7256025E-610.1934041