FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_222_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_222_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6612788
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGACT11000.010.974266
CGGTACA10800.010.85993861
TCGACTG10500.010.5110027
GGCGGTA11850.010.47829559
GCGTCGT8900.09.687325519
ACGGGCG14950.09.45886956
CGCTAGT3000.005888329.19949233
GTCTAAG13350.08.78536844
GCGGTAC13600.08.62315560
GTCGTGC12500.08.277163521
CGGTAAT4200.00117985128.21116219
CATATGC18100.08.19419312
CGCTTAT6151.3846313E-57.85215654
AATCGTA4855.6026835E-47.82360125
ACACACG16550.07.710757352
ACACGGG16200.07.665254
TACATCG14000.07.63822943
CTAAGTA16900.07.55186746
GTTAGAT14800.07.54661941
GAATCGT11455.820766E-117.5319944