FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_222_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6612788
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA2253.476246E-513.80073866
CGATCGG5451.8189894E-1212.65787641
GTCCGAT9200.012.5131911
GCGATCG5800.012.48874240
GTCGAAT5401.2551027E-1011.6284191
ATTCGAA5652.7284841E-1111.598188
GTCGGTT5157.021299E-1011.5155221
GTCCGCT10150.011.3420061
GGCGACT7400.011.3141371
CGCGTAA2450.001095656911.2664267
GTCGTAT6551.8189894E-1211.1845871
GTCGTTT10200.010.6023831
TCGACCG3602.1889078E-510.53816531
GTCGGCT10350.010.4487251
TTCGAAT6807.6397555E-1110.1438429
GGCGTTT10500.09.96721651
GTCGTTA5301.5143814E-79.8731861
CGACCGT3854.5844623E-59.85379532
CGCTAGT2850.0035179699.7923541
CCCTAGT5007.254548E-79.7678731