FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_221_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_221_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30521852
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCCG39700.08.77529352
CTCGATT39050.08.74660749
TCTCGAT41400.08.33284148
CGGTACA48850.08.12084261
TCGATTC45100.07.955770550
ATTCCGT44450.07.75988853
GTCTAAG56800.07.348687644
GGCGGTA52650.07.271298459
ACTAGGC45200.07.249037711
TCACCCG56950.07.147253
TTCTCGA47900.07.12911247
CGATACG36850.06.92848329
GTTTAAT138000.06.77814441
GCGGTAC61950.06.73673160
CGCGGTA18050.06.687886717
GTTTATT184150.06.65258651
ATTCGAA34200.06.45511345
TATTCGC23000.06.4479177
CGATTCC56050.06.40009451
GCGTATA18450.06.356107739