FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_210_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_210_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30997995
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT38300.010.53926849
TCTCGAT42800.08.78564948
CGGTACA51950.08.6981761
GGCGGTA52150.08.66376259
ATTCCGT46050.08.31400453
GATTCCG46450.08.24248852
GTCTAAG57850.08.05039944
TCGATTC49400.08.03145650
GCGGTAC62650.07.762524660
TTCTCGA51150.07.55309647
GTAGTCG31350.07.480925657
TCACCCG61250.07.43397573
GTTTAAT141900.07.40102531
ATCGACT49100.07.09483056
CGGGAAT55350.07.06005761
ACTAGGC36700.06.95445111
CGATTCC60150.06.70917651
GTTTATT188150.06.60166651
ACACACG78550.06.586673352
CTCACTA83950.06.53229767