FastQCFastQC Report
Wed 9 Nov 2016
WTCHG_323579_210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWTCHG_323579_210_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30997995
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG18050.014.90378231
GTCGGTT24550.014.2044541
CGACCGT20450.013.32328932
GTTCGAC22750.012.88707729
GTCCGTT39500.011.8299731
GTCCGCT39500.011.0354231
GTCCGAT36350.010.7445861
TCGACGC28700.010.455626543
GTCGTTT51000.010.3248281
GTCGCAT23850.09.79630851
GTCGGAT40250.09.7034951
CCCGTAT26300.09.6792821
GTCGCTT45000.09.4541691
GTCGGCT46000.09.3244521
TTCGACC30050.09.29701330
AGTTCGA32050.09.14759828
GTCCGAA32250.09.0829231
TCGCGTA13850.08.96813466
GTCGGAA51050.08.9485281
GTCGTAT31700.08.8004881